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Indian Journal of Medical and Paediatric Oncology
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Year : 1996  |  Volume : 17  |  Issue : 3  |  Page : 147-151

Ultrastructural peroxidase reaction in acute unclassifiable leukemias.


Tata Memorial hospital, Bombay, India

Correspondence Address:
S Bajpai
Tata Memorial hospital, Bombay, India

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Source of Support: None, Conflict of Interest: None


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Ultrastructural myeloperoxidase was evaluated in 41 cases of acute leukemias of indeterminate lineage. The morphology, cytochemistry and surface marker studies were inconclusive for any specific lineage and therefore these could not be classified according to the F.A.B criteria. Based on cytochemical and immunophenotyping results we could identify 37 patients with myeloperroxidase(MPO)Negativity along with absent lymphoid and myeloid surface markers(Group A)and 4 patients with low MPO(1-2 )positivity(Group B).Further studies for MPO and platelet peroxidase(PPO)at ultrastructural level were helpful to identify the lineage in 17 of the 41(42.8)of our patients. The need of ultrastructural peroxidase demonstration to identify the nature of morphologically and cytochemically undifferentiated blasts is highlighted.


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