Home | About IJMPO | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Indian Journal of Medical and Paediatric Oncology
Search Article 
  
Advanced search 
 
Year : 2003  |  Volume : 24  |  Issue : 4  |  Page : 6-12

Flt-3 ligand increases the effect of cytokines on CFU-GM colony formation from human cord blood and fetal liver CD34 plus cells


Department of Pediatrics Research Center of Michigan

Correspondence Address:
S Bakhshi
Department of Pediatrics Research Center of Michigan

Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and PermissionsRights and Permissions

Background: Transplantation of primary isolates of human cord blood (CB) and fetal liver (FL) hematopoietic stem cells, and the use of ex vivo expanded progenitor cells from these sources hold promise for the treatment of a number of diseases. In order to fully realize the potential of this method and these cells, an understanding of the expression of hematopoietic growth factor receptors and the function of their ligands is crucial. Flt3 receptor is a member of the tyrosine kinase receptor familyand its ligand is a cytokine involved in hematopoiesis, and may have a significant potential for the expansion of hematopoietic stem cells ex vivo. Methods: We investigated the expression of flt3 receptor in CD34plus cells from CB and FL using RT-PCR andflow-cytometry. We also studied the colony forming potential of CB and FL CD34plus cells in methylcellulose culture using a combination of cytokines that included SCF, IL-3, IL-6, GM-CSF, G-CSF and erythropoietin with and without flt3 ligand. Results: Flt3 receptor mRNA and protein expression were observed in CD34plus cells from CB and FL. After 14 days in methylcellulose culture, there was a statistically significant increase in the number of CFU-GM but not BFU-E or CFU-GEMM colonies, when flt3 ligand was added to the cytokine cocktail. The increase in CFU-GM with flt3 ligand was reduced when anti-flt3 ligand antibody was added to the culture. Conclusions: These observations suggest that flt3 ligand may play a synergistic role with cytokines in augmenting CFU-GM formation from both CB and FL CD34plus cells.


[PDF Not available]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed593    
    Printed38    
    Emailed0    
    PDF Downloaded0    
    Comments [Add]    

Recommend this journal